Mr Dhanasekaran Sakthivel

PhD Student
Department of Biochemistry and Molecular Biology
Monash University

dsak8@student.monash.edu

Research Activities

I successfully completed my PhD from Monash University, Australia in September 2017 under the supervision of A/Prof. David Piedrafita and A/Prof. Travis Beddoe. My research interests are largely directed towards understanding the basis of infection and  immunity. I am looking for an early career opportunity to ameliorate and upgrade my knowledge in the field of infectious diseases.

Techniques/Expertise

  • Demonstrated experience in structural and functional characterisation of proteins involved in complex host-pathogen interplay.
  •  Demonstrated experience in defining the innate immune response to multiple pathogens in
  • animal models.Extensive experience in recombinant proteins production (over 40 gene constructs), biochemical, biophysical characterisation and functional studies.
  • Molecular biology:  Gene cloning and gene expression analysis (mRNA and Protein) and gene expression profiling (qPCR).
  • Protein biochemistry: Affinity purification (IMAC and Pull down), Pull down affinity purification (Protein-protein and Protein-glycan), size exclusion and Ion exchange chromatography (AKTA). Circular Dichroism (CD), Analytical Ultracentrifugation and Glycobiology techniques
  • Protein crystallisation: Setting up crystal screening, cryopreservation and diffraction of protein crystals and structure determination)

Disease Models

  • BALB/c mice for the propagation of Plasmodium berghei, Plasmodium yoleii, Plasmodium falciparum, plasmodium chabaudi
  • Chicken for the New castle disease virus vaccine
  • Sheep and goat for the propagation of  H. contortus immune response 
  • Bovine for the vaccine studies of Theileria annulata 

Genetically Modified Organisms

E. coli

Other members with similar research interests

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Prof James Beeson

Centre for Biomedical Research Burnet Institute SEE FULL PROFILE >

A/Prof Heidi Drummer

Life Sciences Burnet Institute SEE FULL PROFILE >

Prof John Hamilton

Department of Medicine (Royal Melbourne Hospital/Western Hospital) University of Melbourne SEE FULL PROFILE >

Upcoming Events

  • Aug 26

    Student Open Day Hudson Institute of Medical Research

    Hudson Institute of Medical Research and Monash University MORE INFO
  • Sep 4

    Student Open Day Walter and Eliza Hall Institute

    Walter + Eliza Hall Institute MORE INFO
  • Sep 10

    Student Open Day Walter and Eliza Hall Institute

    Walter + Eliza Hall Institute MORE INFO