Research Activities
Interested in designing, developing and testing novel vaccines against HIV
Research Keywords
Researching in the following areas:
Virology, Vaccine development, Mucosal immunology
Techniques/Expertise
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Molecular gene cloning
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Designing and constructing
genetically-stable and replication-competent recombinant viruses as vaccine
vectors
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DNA and RNA extraction,
purification, quantification and analysis using standardised methods
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Sanger DNA sequencing and sequence analysis using BioEditTM and
LasergeneTM softwares
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Transformations, transfections,
protein extraction and purification
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Agarose gel electrophoresis,
SDS-page,western blot analysis and immunofluorescence
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In-vitro and in-vivo virus culture,
purification and titration systems
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Handling mouse models for testing
vaccine immunogenicity and protective efficacy
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Multi-colour flow cytometry on
splenocytes, lymph node cells and peripheral blood mononuclear cells
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ELISpots assay for lymphocyte
activity
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Fluorescent-Target array
Disease Models
Human and mouse models
Genetically Modified Organisms
viruses and bacteria