Mr Khamis Tomusange

PhD Student
Surgery
University of Adelaide

khamis.tomusange@adelaide.edu.au

Research Activities

Interested in designing, developing and testing novel vaccines against HIV

Techniques/Expertise

·         Molecular gene cloning ·         Designing and constructing genetically-stable and replication-competent recombinant viruses as vaccine vectors ·         DNA and RNA extraction, purification, quantification and analysis using standardised methods ·         Sanger DNA sequencing  and sequence analysis using BioEditTM and LasergeneTM softwares ·         Transformations, transfections, protein extraction and purification ·         Agarose gel electrophoresis, SDS-page,western blot analysis and immunofluorescence ·         In-vitro and in-vivo virus culture, purification and titration systems ·         Handling mouse models for testing vaccine immunogenicity and protective efficacy ·         Multi-colour flow cytometry on splenocytes, lymph node cells and peripheral blood mononuclear cells ·         ELISpots assay for lymphocyte activity ·         Fluorescent-Target array

Disease Models

Human and mouse models

Genetically Modified Organisms

viruses and bacteria

Other members with similar research interests

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Mr Sj Shen

Department of Medicine - Monash Medical Centre Monash University SEE FULL PROFILE >

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Faculty of Medicine, Nursing and Health Sciences Monash University SEE FULL PROFILE >

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