Techniques/Expertise

·         Molecular gene cloning ·         Designing and constructing genetically-stable and replication-competent recombinant viruses as vaccine vectors ·         DNA and RNA extraction, purification, quantification and analysis using standardised methods ·         Sanger DNA sequencing  and sequence analysis using BioEditTM and LasergeneTM softwares ·         Transformations, transfections, protein extraction and purification ·         Agarose gel electrophoresis, SDS-page,western blot analysis and immunofluorescence ·         In-vitro and in-vivo virus culture, purification and titration systems ·         Handling mouse models for testing vaccine immunogenicity and protective efficacy ·         Multi-colour flow cytometry on splenocytes, lymph node cells and peripheral blood mononuclear cells ·         ELISpots assay for lymphocyte activity ·         Fluorescent-Target array