Dr Julianne Bayliss
Research Fellow
Molecular Research and Development
Victorian Infectious Diseases Reference Laboratory
julianne.bayliss@mh.org.au
Research Activities
- Characterisation of viral variants which are associated with specific clinical outcomes following chronic infection with HBV
- Pathogenesis of HBV associated hepatocellular carcinoma
- Monitoring of the response to antiviral treatment through the use of next generation sequencing techniques
Techniques/Expertise
- Molecular biology (traditional, real time, competitive and nested and triple nested PCR techniques)
- Cell culture (media preparation, viral transfection and cell stimulation/treatment)
- Nucleic acid extraction (RNA and DNA from fresh, frozen, fixed and archival tissues)
- Sequencing of PCR products (Sanger and Next Generation ultra deep- Roche 454 platform, Illumina MiSeq and QIAGEN PyroMark)
- Matrix assisted laser desorption/ionisation time of flight mass spectrometry (genotype analysis, microbial identification and cytokine quantification techniques)
- Laser capture microdissection (PALM System)
- Histology (sectioning of frozen and fixed tissue, immunoperoxidase and immunofluorescence techniques and staining of tissue)
- Sample processing (blood, bronchoalveolar lavage (BAL) fluid, urine, fresh and frozen tissue, including handling of infectious materials)
- Statistical analysis (STATA12) (Basic univariate analysis of parametric and non parametric data, multivariate analysis including multiple linear and logistic regression modelling, ROC and survival analysis)
