Mr Wilson Gauci

Honours Student Graduate
Department of Physiology, Anatomy and Microbiology
La Trobe University

WilsGauc10@gmail.com

Research Activities

Attempted generation of an artificial res-site hunter transposon using restriction cloning techniques. Creation of theorised clone constructs in silico using Geneious Prime. Transposition assays of the Tn502 res-site hunter transposon to observe transposition frequencies in the res-site of IncP plasmids and its insertional orientation.

Techniques/Expertise

Expertise
  • Bacterial transposons (res-site hunter transposons)
  • Bacterial plasmids
  • Molecular Cloning & DNA analysis
Techniques
  • Restriction/Conventional Cloning in E.coli
  • Bacterial culturing
  • Conjugative mating
  • Transposition assays
  • Plasmid extractions (Minipreps)
  • Gel electrophoresis
  • PCR and Colony PCR
  • DNA sequence analysis & in silico manipulation using Geneious Prime
  • PCR primer mapping

Genetically Modified Organisms

Using E. coli DH5a & P. aeruginosa PAO9503 strains

Other Lab Members

Miss Cassandra Stanton

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