Dr Nicole Mifsud

Group Leader
Department of Biochemistry and Molecular Biology
Monash University

+61 3 9902 9311
nicole.mifsud@monash.edu

Latest News

Recently appointed Group Leader Clinical Immunology, 2017

Research Activities

My research expertise encompasses human leukocyte antigen (HLA) immunology andimmunogenetics, transplantation immunology, viral immunology and T cell biology. Myresearch interests explore how the antigen-specific T cell repertoire, shaped by previousexposure to environmental pathogens (thereby forming a historical immunologicaltemplate), is able to influence future immune responses through TCR cross-reactivity.The TCR/HLA/peptide complex is critical for immune reactivity that is imperative forpathogenic control or clearance but is also pertinent to many human disorders (i.e.autoimmunity, allergy, cancer) and therapies for end-stage disease (i.e. transplantation).Therefore, TCR cross-reactivity may underpin or contribute to immune mechanisms thatdrive susceptibility to numerous human disorders via inappropriate T cell responses. Mycurrent areas of investigation spans across a spectrum of relevant biology and clinicalmedicine via autoimmunity, respiratory disorders, drug allergy and solid organtransplantation. The long term clinical impact of my research has the potential to be far-reachinggiven the core nature of immunology involved including translatable outcomesin aiding clinicians to tailor the individual patient clinical management, influenceimmunotherapeutic approaches and potentially altering drug design.
Key research areas:
  • Transplantation immunology
  • Viral immunology
  • T cell cross-reactivity
  • HLA immunogenetics
  • Drug hypersensitivity
  • Antigen processing and presentation

Techniques/Expertise

Human T cells (lines and clones)
Human DC cultures
Human T cell receptor repertoire identification
Transfection of cell lines
Immunological cellular assays
Generation of MHC/peptide class I tetramers
Flow cytometry
Protein expression and purification
Molecular biology
Immunoproteomics (RP-HPLC and mass spectrometry)

Collaborations

A/Prof Tom Kostimbos, Prof John Wilson (Lung Transplantation, Cystic Fibrosis), The Alfred Hospital
Prof Robyn O'Hehir (Drug-induced allergy), The Alfred Hospital
Prof Anthony Schwarer (Haematopoetic Malignancies), Box Hill Hospital
Dr George Grigoriadis (Haematopoietic malignancies), The Alfred Hospital, Monash Health and Translation Precinct
Dr Raffi Gugasyan, Burnet Institute
Prof Weisan Chen, La Trobe University
Prof Scott Burrows, QIMR Berghofer Medical Research Institute
Prof Katherine Kedzierska (Influenza), PDI, University of Melbourne
Prof Frans Claas (Transplantation), Leiden University Medical Centre, The Netherlands
A/Prof Lloyd D'Orsogna (Transplantation), Fiona Stanley Hospital, WA
Prof Alexandra Sharland (Transplantation), University of Sydney, NSW
Dr James Yun (Drug-induced allergy), Royal North Shore Hospital, NSW
Prof Patrick Kwan (Drug-induced allergy), Royal Melbourne Hospital, The Alfred Hospital
A/Prof Stephanie Gras (T cell cross-reactivity), Monash University
Prof Anthony Purcell (Immunoproteomics), Monash University

Disease Models

Solid organ transplantation: lung, kidney
Haematopoietic malignancies
Cystic Fibrosis
Drug-induced allergy

Genetically Modified Organisms

Cell lines (C1R, 721.221, K562, T2) transfected with specific HLA class I molecules

Other Lab Members

Dr Louise Rowntree (post-doc)
Ms Heleen van den Heuvel (visiting from Leiden University Medical Centre, Leiden, The Netherlands)
Ms Kirty Pandey (PhD student, 2017)
Ms Kerry Mullan (PhD student, 2018)
Ms Johanna Tuomisto (PhD student, 2018)
Mr Shawn Goh (Honours student, 2017)
Ms Stephanie Davies (Honours student, 2018)
Ms Grace Khuu (Honours student, 2018)

Research Images

Other members with similar research interests

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Upcoming Events

  • Apr 22

    Malaria Experimental Genetics workshop

    Mahidol University Faculty of Tropical Medicine, Bangkok, Thailand MORE INFO
  • Apr 29

    International Day of Immunology

  • May 7

    VIIN Careers Evening 2018

    Woodward Conference Centre MORE INFO